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The Impact Of Internet On Traditional Media - 1652 Words

The Impact of Internet on Traditional Media News Xiaoran Yang Z5047673 ï  ¬ Introduction The Internet has become one of the most popular mediums that facilitating a variety of communication and information-sharing tasks from the past decades. Internet has an effect on the use of traditional media news at the same time. The advent of Internet has caused a challenge to traditional media news. This Essay will discuss and argue the decline of usage of traditional media news (this essay will only focus on newspaper news). It will look at the current situation of the usage of traditional media news as well as Internet. Analysis of the causes of the current situation will also include in this essay. In addition, providing the viable alleviation strategies, which focus on how traditional media news promote the usage will be provided. The evaluations of strategies are the last part of the main content. Case studies are also provided to assist the research in this essay. ï  ¬ The Decline of Usage of Traditional Media News Over the last decade, the development of technologies in the area of communication has been multiple rapidly taken place. Internet has become one of the most popular mediums to serve information-sharing worldwide. The statistics from internet users (2016) shows that around 40% of the world population has an internet connection today. However in 1995, it wasShow MoreRelatedThe Impact of Internet on Traditional News Media2001 Words   |  9 PagesTHE   IMPACT   OF   INTERNET   ON   TRADITIONAL   NEWS   MEDIA    1    The impact of the Internet on traditional news media Lingyan Chen Fairleigh Dickinson University May 6th, 2013 THE   IMPACT   OF   INTERNET   ON   TRADITIONAL   NEWS   MEDIA    Abstract The study discusses the impact of the Internet on traditional media over time. Media research suggests that Internet would be allowed to exist with traditional news media at the same time, rather than killing off the traditionalRead MoreMedia, Television, And Media1404 Words   |  6 Pagesthe use of technology and media. Media is defined as â€Å"the means of communication as radio and television, newspapers, and magazines, that reach or influence people widely† (Media, n.d.). Not only is media used in public relations, but it is used in the daily lives of a majority of the world’s population. There are two types of media , traditional media and new media. 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Information once contained in massive volumes at libraries or in private collections is now available by typing words into a search engine and clicking â€Å"search.† One must no longer pick up a phone to call a friend, relative or colleague; e-mail, instant messaging, Skype and the like, have enabled people to communicate in non-traditional ways and across boundaries previously inaccessible.Read MoreInternet Impa ct On The Internet866 Words   |  4 PagesThe internet is the newest mass media and has the potential to change human society. It has given us the ability to access almost all of human knowledge in an instant. It has also allowed the constant connection between friends and family. As technologies go it may well be the most influential in human history. 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Never Scared The Cultural Significance of Chris Rock Free Essays

string(60) " experience less stress and live a healthier life\(Waite\)\." Standup comedians exemplify the trans-generational nature of our culture. With their ability to fully embody all of societies diverging values, while still always grazing the edge of change, they serve as the conscience of the people.   As Lawrence Mintz argues, comics are licensed to say the unspeakable because they have the pity of the audience; they use the power of laughter to unite communities and tread societies shade of gray, and the most successful of them exercise a full awareness in the art of rhetoric. We will write a custom essay sample on Never Scared: The Cultural Significance of Chris Rock or any similar topic only for you Order Now    Mintz points out that comedians use these weak pity warranting social positions to actually empower themselves.i One of the top standup comedians known for this today is Chris Rock; he uses rhetoric to persuade his audience into finding humor in some of the darkest aspects of our society.   Chris Rock uses rhetoric in his standup Never Scared to persuade his audience to adopt his views, while at the same time reversing the pity warranting image that Mintz’s claims gives a comic his license to speak freely.   Both of these personal intentions of Rock’s in his stand up are dependent solely on how well he connects to the core values of his audience. Rock is credited for being best able at connecting the disintegration of family and relationship values of Blacks. In Never Scared, Chris Rock discusses the deteriorating values of Black America and how these values are affecting black culture in America. He utilizes theories in social family structure with the African American family today and establishes himself as a role model for the black culture. He also sarcastically undermines the institution of marriage and the battle of the sexes in an intelligent and witty manner. Not only is he socially aware of all of these things that I have mentioned, he is also aware of the embarrassing acts from his race whom he spitefully labels â€Å"niggas.† Allow me to expand on the issues Chris Rock brings up in his comedy routines. The days of funny schtick and prat falls are basically over. Through the years, much more substance has come to the attention of America when it comes to stand-up comedy. Lawrence Mintz states the following concerning this phenomena on page 72; â€Å" Clearly it is a popular art that is central to American entertainment, but in the universal tradition of public joking rituals it is more than that as well; it is an important part of the nation’s cultural life.†i Chris Rock is an excellent example of this statement. In Never Scared, Rock opens his routine with confidence and mentions his daughter, alluding to the reason why he hasn’t been on the road in so long. â€Å"It’s amazing when you have a girl†¦ It’s eye opening, because I realize, I’m the man in her life.   My relationship with my daughter is going to affect her relationship with men for the rest of her life.   Every man in here has dated a woman with some daddy issues.   That s@%$# ain’t fun ok.   She’s giving you a hard time for some s@%$# her daddy did in 1969.† (Chris Rock, 2006) Ever the sarcastic encourager, Rock sets up an example that needs to be revisited in the black community. He’s sensible and funny here but he is also alluding to something important. The black man in today’s culture needs to understand the importance of bringing up baby girls. He states that sometimes he picks her up out of her stroller, looks at her and that’s when it hits him;   Ã¢â‚¬Å"My job in this life now is to keep my baby off the pole!† Of course he is referring to the ever-ominous pole found in every strip club in the world. This is profound wisdom for the deadbeat dads out there who aren’t with their daughters. Some fathers are right in the room but to busy watching the game to pay any attention to the direction she might be headed if he doesn’t start to get to know his little girl who is growing up. It’s interesting to note that an article by Kathryn Edin and Laura Lein almost discuss the same issue.ii With the rise of single mothers in urban areas and their struggle to make ends meet, we have to wonder what the sociological and psychological implications for the child are growing up in an environment without a father. Many of these children will grow up to be drug dealers, strippers and prostitutes in their struggle to get away from the economic prison they were unwillingly placed in at birth. Speaking of birth, let’s examine Rock’s use of abortion and marriage. Nilsen discusses the important element of sexism in comedy routines in his article; â€Å"Sexist humor, which makes fun of the real or imagined characteristics of males and females, is seen in the oldest myths, fairy tales, folk tales, nursery rhymes, and sacred writings. Because jokes are a kind of shorthand, creators do not start with a whole new cast of characters for each joke; instead they rely on familiar scripts that include exaggerations and stereotypes. This enables listeners to fill in the details from the material that their minds have already absorbed from the popular culture.†iv Throughout Rock’s comedy routines, he brings to light the battle of the sexes concerning abortion and marriage. He discusses the two options that a man has when he finds out his girlfriend is pregnant; â€Å"Wow! I’m so happy! I love you so much.† Or the ill-fated; â€Å"So, whatcha gonna do?† Once again he silently stabs at the lack of responsibility concerning the man in this issue. He goes on to discuss the fact that the decision for abortion is made between the woman and her girlfriends. It is a sad but true commentary on the disintegration of society in general but Chris seems to be directing it toward black culture specifically. There are strong arguments for the fact that married couples experience less stress and live a healthier life(Waite). You read "Never Scared: The Cultural Significance of Chris Rock" in category "Essay examples"iii Rock equates marriage to simple transference; â€Å"When you’re single, you wanna kill yourself. When you’re married, you wanna kill your spouse!† He discusses the problem of unhappily married men and their addiction to strip clubs. He adds that reasons for this are that women are domineering. In a funny little clip, Chris talks about the ho convention and glass heels. Then he brings up the fact that a wife is there to provide for you and be there for you but if you bring a pair of glass heels in the house, it will cause all sorts of problems. As you can see, he strengthens his views of the black man being the wife’s pet or the one that’s supposed to do everything for her by using these jokes. He also portrays the man in the abortion bit as not having a say in anything that happens with the child. He portrays the woman’s friends at a higher level than her own boyfriend and the father of her child. He jokes about strip clubs and infidelity but he still holds to the role model persona. He stands by his conviction of not cheating on your spouse and once again cements his position in black culture. Voicing his family values opens the audience up for his personal opinions about society.   Rock’s humorous perspective on life and his personal opinions about society mark the defining line between him as an individual and the family values he has affirmed to gain the audience’s trust. In his essay, Standup Comedy as Social and Cultural Mediation, Mintz explains the justification behind the stand up comedian’s license to speak freely. Considering the fact that Chris Rock is an African American, he has free reign to speak out on the problems with African- Americans.   As he displays his disgust with black stars like the child molesting, wanna-be- white Michael Jackson, he flows freely into the R-Kelly incident with the young girl on video. We all know that he is using these incidents as well as the Kobe Bryant rape case and the OJ Simpson case because they are excellent material for comedy. But, it’s also obvious that he uses these men as examples for negative black role models in America as well. It makes one wonder if he is warning his black audience of the path of self-destruction they may be on together. After all, great men like Sydney Poitier or James Earl Jones didn’t do things like this to embarrass the black community. Personally, I believe that his most compelling routine is the â€Å"I hate Niggas† routine. The courage that it takes for a black man to stand up and say â€Å"Everything white people don’t like about black people, black people hate even more. I love black people but I hate niggas.† He jokes about the fact that every time black people try to get together and have some fun like going to a movie, some â€Å"nigga† pulls out a gun and shoots at the screen. It is most powerful when he utilizes this rhetoric in the presence of a more affluent black community. By performing in D.C., Rock intentionally markets himself towards the wealthiest blacks in the United States, giving him all the more power once he is able to form a community within the room.   But, this community Rock creates must be structured on some form of values with which he knows everyone can basically agree, and that will create an atmosphere of familial comfort.   Rock does this perfectly in his stand up, and the structure can be noted from start to finish. We can also observe the moral erosion of rap music. It used to be a positive influence in the black community. Chris Rock has played an important role in promoting rap music. Although, in Never Scared, He mentions the fact that it keeps getting harder to defend this popular urban genre. He states that it used to be easy to defend groups like Grand Master Flash because they represented the black urban culture as a whole. Then he goes on to state that he hates to defend it now because of lines such as the famous line from Li’l Jon; â€Å"To the windows, to the walls, ‘til the sweat drips from my balls.† These lyrics can’t be defended. They do not spell anything positive for black society and the song certainly shows no respect for black women whatsoever. Granted, there were some pretty vulgar lyrics in the early days of rap also but not to the extent of today. If we observe the work of Chris Rock, a strong argument can be made that he has created some of the most powerful rhetoric concerning the degradation of his own race in America. He stands by his values and doesn’t squirm under the microscope like some stars. He has used his influence to create a teachable understanding of the social inequalities that occur in America. But, more than this he has made it simple for the average black family, or any family, to understand the major social issues surrounding black culture today. I think the most important thing that Chris Rock has done for the black culture and every culture in America is created a vital understanding for accountability within our own cultural social dynamic. I have heard many people say that Chris Rock is a comedian, but he is an activist as well. Others say that his comedy is racially motivated against the white population of America. Still, some just won’t watch him or listen to him because of his language usage. There are a number of labels we can put on this man and he allows us to do so liberally. Chris Rock may have missed his true calling as a social scientist. Maybe one-day comedians like Rock will receive honorary PhDs for their body of work in stand up comedy but for now, he’s just a great comedian. iv Nilsen A.P and Nilsen D.L.B (2000) Encyclopedia of the 20th century American Humor.Gender Humor. Phoenix, AZ: Oryz Press, pp.170-174. i Standup Comedy as Social and Cultural Mediation. Lawrence E. Mintz. American Quarterly, Vol. 37, No. 1, Special Issue: American Humor. (Spring, 1985), pp. 71-80. ii Work, Welfare, and the Single Mothers’ Economic Survival Strategies. Kathryn Edin and Laura Lein. American Sociological Review. iii Does Marriage Matter?. Waite, Linda J. Demography. Vol. 32, No. 4, November 1995. iv Nilsen A.P and Nilsen D.L.B (2000) Encyclopedia of the 20th century American Humor.Gender Humor. Phoenix, AZ: Oryz Press, pp.170-174. Quotes from Chris Rock came from his stand up routine Never Scared. 2006. How to cite Never Scared: The Cultural Significance of Chris Rock, Essay examples

Female Genital Mutilation Essay Example For Students

Female Genital Mutilation Essay Female Genital MutilationImagine a young girl; the harsh African sun is kissing her bronzed skin. The warm golden sand tickles her petite and tattered feet. The immense gold earrings she wears beats against her slender neck. Her stature is of a queen, yet she walks to an uncertain death. She stands in front of a small hut, or a tent. She glances back and sees the majestic sun that had once kissed her neck now set and somewhat leave her abandoned. She exists alone in front of that diminutive hut or tent and out comes a man. He is exhausted and is ready to go home to his companion and his supper. He looks a bit annoyed that she has come so late. His hands are stained with a ruby tint and his clothes the same. He motions the young girl in. Hesitantly, she makes small and meager steps to the entranceway. She steps into a minute room with little or no lighting. She stares upon two women and a rusty table that holds the screams of the girls that went before her. The man motions her to sit in the table. She slowly places her body on the stained and rusty table. She is a bit afraid that the table will not hold under her weight; nevertheless, she is held up. The man places his cold and clammy hands on her collarbone and pushes her back to the table. As she lies there she looks to her left and sees his instruments; a bloody and rusty razor blade. She sighs with relief. She has heard that a razor blade is the best instrument to use. She knew of women that had to take a piece of glass. She has prayed for courage and strength, yet it does not seem to arrive. The man runs his hands down the sides of her body. Has he pushes her skirt up he looks at her and says to her, Dont move. He opens her legs and begins to operate. The glare from the poor lighting obstructs his view, but he continues any way. The heat has gotten to him and he is not as awake as he was in the morning. He blinks to regain some concentration and he takes his blade in his hands. He thinks about cleaning the blade first but the thought immediately escapes from his mind. He does not want to waste any more time on this girl. The young girl sees the man raise his blade and she begins to squirm. With their hands, the women hold her legs to gain sight of his target. As the sun finally sets and the night creeps upon them, the earth and all its inhibitors are disrupted by a shrill. The screams bellow out into the night and echoes in the stars. Moments later the young girl stumbles out of the hut or tent. She is now a woman. Imagine this happening to over 100 million women around the world. This is called Female Genital Mutilation or FGM. It is an invasive procedure performed on girls before puberty. Part or all the clitoris is surgically removed leaving them with reduced or no sexual feeling. FGM is very emotional because many women do not have the confidence to talk about their feelings and what happens to them during the circumcision. The stories that some women have are very gruesome and ex tremely painful. Yet if the procedure is not done, the women have to live with being called names and being rejected. The term FGM covers three main varieties of genital mutilation: Sunna circumcision, Clitoridectomy, and Infibulation. Sunna in Arabic means tradition and the Sunna circumcision consists of the removal of the prepuce and/or the tip of the clitoris. Clitoridectomy, which is also call excision, is the removal of the entire clitoris (both prepuce and glans), and the removal of the adjacent labia. Infibulation is also called pharaonic circumcision. This is the most extreme form and it involves removal of the clitoris, the adjacent labia (majora and minora), and the joining of the scraped sides of the vulva across the vagina, where they are secured with thorns or sewn with catgut or thread. A small opening is kept to allow passage of urine and menstrual blood. An infibulated woman must be cut open to allow intercourse on the wedding night and is closed again afterwards to secure fidelity to the husband. .ufbbf571c47992bbfe0156c7d866a203e , .ufbbf571c47992bbfe0156c7d866a203e .postImageUrl , .ufbbf571c47992bbfe0156c7d866a203e .centered-text-area { min-height: 80px; position: relative; } .ufbbf571c47992bbfe0156c7d866a203e , .ufbbf571c47992bbfe0156c7d866a203e:hover , .ufbbf571c47992bbfe0156c7d866a203e:visited , .ufbbf571c47992bbfe0156c7d866a203e:active { border:0!important; } .ufbbf571c47992bbfe0156c7d866a203e .clearfix:after { content: ""; display: table; clear: both; } .ufbbf571c47992bbfe0156c7d866a203e { display: block; transition: background-color 250ms; webkit-transition: background-color 250ms; width: 100%; opacity: 1; transition: opacity 250ms; webkit-transition: opacity 250ms; background-color: #95A5A6; } .ufbbf571c47992bbfe0156c7d866a203e:active , .ufbbf571c47992bbfe0156c7d866a203e:hover { opacity: 1; transition: opacity 250ms; webkit-transition: opacity 250ms; background-color: #2C3E50; } .ufbbf571c47992bbfe0156c7d866a203e .centered-text-area { width: 100%; position: relative ; } .ufbbf571c47992bbfe0156c7d866a203e .ctaText { border-bottom: 0 solid #fff; color: #2980B9; font-size: 16px; font-weight: bold; margin: 0; padding: 0; text-decoration: underline; } .ufbbf571c47992bbfe0156c7d866a203e .postTitle { color: #FFFFFF; font-size: 16px; font-weight: 600; margin: 0; padding: 0; width: 100%; } .ufbbf571c47992bbfe0156c7d866a203e .ctaButton { background-color: #7F8C8D!important; color: #2980B9; border: none; border-radius: 3px; box-shadow: none; font-size: 14px; font-weight: bold; line-height: 26px; moz-border-radius: 3px; text-align: center; text-decoration: none; text-shadow: none; width: 80px; min-height: 80px; background: url(https://artscolumbia.org/wp-content/plugins/intelly-related-posts/assets/images/simple-arrow.png)no-repeat; position: absolute; right: 0; top: 0; } .ufbbf571c47992bbfe0156c7d866a203e:hover .ctaButton { background-color: #34495E!important; } .ufbbf571c47992bbfe0156c7d866a203e .centered-text { display: table; height: 80px; padding-left : 18px; top: 0; } .ufbbf571c47992bbfe0156c7d866a203e .ufbbf571c47992bbfe0156c7d866a203e-content { display: table-cell; margin: 0; padding: 0; padding-right: 108px; position: relative; vertical-align: middle; width: 100%; } .ufbbf571c47992bbfe0156c7d866a203e:after { content: ""; display: block; clear: both; } READ: geology a science of lies Essay Though FGM is practiced all over the world, it is most prevalent in Africa. According to the State-Agency for International developments Intra-Agency working Group on Female genital mutilation, at least forty percent of Nigerian women are victims of FGM. In Africa it is practiced in the majority of the continent including Kenya, Nigeria, Mali, Upper Volta, Ivory Coast, Egypt, Mozambique, and Sudan. A variety of forms of FGM is practiced in Middle Eastern countries: the two Yemens, Saudi Arabia, Iraq, Jordan, Syria, and Southern Algeria. FGM has found its way into the United States and Europe because of immigration. Although FGM is illegal in these countries, the ritual is being practiced secretly. Some claim that FGM is a religious tradition, however, there has been no proof from both of the dominant religions, which is Christian and Muslim. In most African countries the reason for the practice of FGM has nothing to do with inter-sexuality, but more of traditional beliefs. The process is done in unsanitary conditions in which a midwife uses unclean sharp instruments such as razor blades, scissors, kitchen knives, and pieces of glass. These instruments are frequently used on several girls in succession and rarely cleaned, causing the transmission of variety of viruses such as the HIV virus, and other infections. Antiseptics techniques and anesthesia are generally not used, or for that matter, heard of. This is just like your doctor uses one instrument on all of patients without any sterilization procedure. Besides of the obvious initial pains of the operations, FGM has long-term physical, sexual, and psychological effects. The unsanitary environment under which FGM takes place causes infections of the genital area and surrounding areas. Some can have primary fatality as a result of shock, hemorrhage or septicemia. Long-term consequences are sexual fidgety, genital malformation, delayed menarche, chronic pelvic complications, recurrent urinary retention and infection, and an entire range of obstetric complications where the fetus is exposed to a range of infectious diseases as well as facing the risk of having its head crushed in the damaged birth canal. In these cases, an infibulated woman is opened further to insure a safe birth of her child. In various cultures there are justifications for practicing the dangerous practice. A girl who is not circumcised is considered unclean by the local villagers and by that is not worthy of marriage. A girl that does not have her clitoris removed is considered a great danger and ultimately fatal to a man if her clitoris touches his penis. Some have said, She loses only a little piece of the clitoris, just the part that protrudes. The girl does not miss it. There is hardly any pain. Womens pain thresholds are so much higher than mens. Others have said, The parts that are cut away are disgusting and hideous to look at. It is done for the beauty of the suture. Many women of the local villages tha t FGM takes place say that it is a tradition and they do not want to be the ones to break a tradition. Family honor, cleanliness, protection against spells, insurance of virginity and faithfulness to the husband, or simply terrorizing women out of sex are sometimes used as excuses for the practices of FGM. This practice has been committed for years now. It was said to begin in the Egyptian times and has grown from there. About two million women a year undergo a knife, a shard or a piece of broken glass to uphold this barbaric tradition. Of this shocking number, fifteen percent will die as a direct result of this practice. Of those that survive, they will have to live with infections, hemorrhaging, bladder, kidney, and urinary disorders, extreme complications during intercourse and birth and a loss of sexual sensitivity. I decided to talk about this because this is something that I can relate deeply too. No, I am not a victim of Female genital mutilation, but I am a female and just the mere contemplation of this excruciating practice happening to someone my age is heartbreaking. I was first introduced to this problem when I was a junior in high school. We did a segment on traditions throughout the world, and genital mutilation came up. I became interested in the female aspect of genital mutilation. I was reintroduced to the subject of female genital mutilation my first year in college. I worked in the media resource center and a young woman was also interested in FGM. We exchanged information and from then on I have been passionate about the subject. To think that somewhere in this world, even after all of the technology that we have experienced and discovered, a dangerous and painful unsanitary pr5actice is actually being practice is very sad. I honestly believe that official across the world should pay close attention to this tradition that is hurting at least two million women a year and killing fifteen percent of that two million. These women are our mothers, our friends, our daughters, our sisters, or they can even be us. I understand that it is important to hold on to traditions and keep the roots of your ancestors close at hand, but we must consider a different approach once the tradition starts taking away women across the world. Studying this has opened m y eyes and made me realize that the world is still grieving and searching for justice. I hope soon that the world finds it.

Macbeth and the Tragedy Element free essay sample

An analysis of Shakespeares Macbeth and how it differs from other Shakespearean tragedies. Macbeth is one of Shakespeares most well-known tragedies. This paper highlights the difference of this play to some of his other famous plays. The play is very short compared to other plays. Shakespeare uses the play to mimic contemporary issues that were of concern in his time. But unlike some Shakespearean plays, Macbeth is a hero and also a villain. This paper takes a look at these issues. Macbeth is based on the life of the king of Scotland. Macbeth was the governor of Moray and killed a man named Duncan in 1040 in a fight. Macbeth was probably of royal blood. Macbeths wife was a royal she was the granddaughter of Kenneth III. Kenneth III was overthrown by one of Duncans ancestors. Macbeth was eventually defeated in 1054. A man named Malcom killed Macbeth in a fight and sat on the throne as Macolm III. We will write a custom essay sample on Macbeth and the Tragedy Element or any similar topic specifically for you Do Not WasteYour Time HIRE WRITER Only 13.90 / page It has been said that Shakespeare probably adapted the story from versions told by Raphael Holinshed and Hector Boece (Macbeth).

Free Essays on Carpe Diem

Carpe Diem Speech â€Å"Yesterday is our past – tomorrow is our future – but today is a gift†¦ that’s why it’s called the present.† It’s a gift! Right now, right this second, just look around – you have been given the gift of life today. But have you ever thought about the fact that you may not be so blessed tomorrow? Our future in this world is not guaranteed, therefore make the most out of today. There was a theme widely used in the 16th and 17th century that urged people to do just that: carpe diem. I would like to share with you this evening some facts on carpe diem, including its meaning, history, and some examples of applying the phrase. Carpe diem is from the Latin phrase â€Å"seize the day.† It is a descriptive term for literature that urges readers to live for the moment. Encyclopedia.com gave us this definition, but it has a much deeper meaning. It means that one should take advantage of opportunities with wisdom and care. It means that when you get the chance to do something crazy – do it! Ok – so think of the one thing you want in life. Now, make that into one word, just one word. At the same time lets all scream that one word that you dream of.. 1, 2, 3 ..! It’s understanding what we can do – the very best we can do. It’s being ruthless, being resourceful, and being happy. There are many ways we can personally define the phrase carpe diem, but where exactly did it come from? Love poetry from the 16th and 17th century started the phrase carpe diem. One professor from the University of Arkansas’s reviews carpe diem’s history on the school’s website. The phrase itself turns out to be from a poem by Horace, a great lyric poet who had studied in Rome and Athens. However, it is best exemplified by a familiar stanza from Robert Herrick’s â€Å"To the Virgins, to Make Much of Time†: Gather ye rosebuds while ye may, Old time is still a-flying; And this same flower that smiles today, ... Free Essays on Carpe Diem Free Essays on Carpe Diem Carpe Diem Speech â€Å"Yesterday is our past – tomorrow is our future – but today is a gift†¦ that’s why it’s called the present.† It’s a gift! Right now, right this second, just look around – you have been given the gift of life today. But have you ever thought about the fact that you may not be so blessed tomorrow? Our future in this world is not guaranteed, therefore make the most out of today. There was a theme widely used in the 16th and 17th century that urged people to do just that: carpe diem. I would like to share with you this evening some facts on carpe diem, including its meaning, history, and some examples of applying the phrase. Carpe diem is from the Latin phrase â€Å"seize the day.† It is a descriptive term for literature that urges readers to live for the moment. Encyclopedia.com gave us this definition, but it has a much deeper meaning. It means that one should take advantage of opportunities with wisdom and care. It means that when you get the chance to do something crazy – do it! Ok – so think of the one thing you want in life. Now, make that into one word, just one word. At the same time lets all scream that one word that you dream of.. 1, 2, 3 ..! It’s understanding what we can do – the very best we can do. It’s being ruthless, being resourceful, and being happy. There are many ways we can personally define the phrase carpe diem, but where exactly did it come from? Love poetry from the 16th and 17th century started the phrase carpe diem. One professor from the University of Arkansas’s reviews carpe diem’s history on the school’s website. The phrase itself turns out to be from a poem by Horace, a great lyric poet who had studied in Rome and Athens. However, it is best exemplified by a familiar stanza from Robert Herrick’s â€Å"To the Virgins, to Make Much of Time†: Gather ye rosebuds while ye may, Old time is still a-flying; And this same flower that smiles today, ...

Compare between the puplic transport in Oslo city (norway) and western Essay

Compare between the puplic transport in Oslo city (norway) and western australia - Essay Example The city of Perth basically runs the CAT buses around the city to provide a free service to visitors and also to reduce the regular traffic. (Public Transport, Perth). Trains: the train service in Perth also very much advanced and well controlled. A traveler need to have a SmartRider to have an access in the FTZ using any of the train services, but, it is free of charge if someone having a journey within the FTZ boundaries. The tickets for the trains can be purchased from the machines at the stations or one can validate the SmatRider Ticket before entering the train. The public transport cost is very high in Perth for the best quality. Most of the revenue in the transport department comes from the individualized marketing program. However, the fare box revenue from these marketing programs is not enough. The private bus operators require approximately sixty two percent of additional fare. (McClintock 287) The Oslo Public transit system is basically comprised of buses, trams, ferries, local and intercity trains and also an extensive metro system called T-bane. A single ticket allows the traveler to use the full transit system for one hour. This is a very convenient way to roam the city around the city in summer. By this route a traveler can take a trip to the several Islands. Most of the boats depart from the piers outside the Oslo City Hals. (Henrik â€Å"Orientation and Sighseeing† page number has been omitted in the source) For this specific study it has been seen that for both the cities the public transport system is very much advances and run with a very well designed structure. However, the free ride is pretty expensive for the transport department and that part should be taken care of to make it a profitable

Qualitative Research Essay Example | Topics and Well Written Essays - 750 words - 2

Qualitative Research - Essay Example Hubbard reckons the gendered attitudes and beliefs to arise from a merging of the students’ school, family and community ethos. Her results prompt the author to question â€Å"the wisdom of Ogbu’s undifferentiated treatment of the African American student population.† (p. 605). In her study, Hubbard found that a combination of ethnicity, class and gender determined the academic achievements of her subjects whereas Ogbu had asserted that under-achievement in schools by minority students was influenced essentially by cultural factors, and not by any inherent deficiency of racial, genetic or academic traits. According to Hubbard, â€Å"Recognizing the salience of gender in constructing academic identities extends the work Ogbu started and corrects an oversight that plagues his work.† (p. 606). Hubbard has chosen to focus on cases of successful minority students rather than look to explain the reasons for minority students’ failure. Through this study, Hubbard has tried to find answers to several questions including how to â€Å"account for the relative success of African American students who not only stay in school but also do well and become eligible for college?† What made the subjects of her study â€Å"not assume an ‘oppositional stance’, as Ogbu’s theory predicts?† and â€Å"why are female African American high school students typically more academically successful than their male counterparts?† The gender issue in the educational field is not a new phenomenon and it is not restricted to any ethnic group. In fact, low aspirations coupled with poor examination results in boys seem to be a global problem. Furthermore, gender-based differentiation vis a vis over-achieving girls and under-achieving boys has become a common feature in recent years so that experts in education are strongly advocating specific strategies intended to improve educational outcomes for

Symbolism in the Short Story Research Paper Example | Topics and Well Written Essays - 250 words

Symbolism in the Short Story - Research Paper Example That is the main theme of the short story, talking versus communicating. Both parties eventually became frustrated with the direction their conversation is heading, which then leads both of them to put more walls between them, thus aggravating the situation. For example, the girl looked at the hills and mentioned they are lovely, but the man replied pertaining to getting another drink. Then the American said â€Å"The beer’s nice and cool,† to which the girl replied â€Å"It’s lovely.† (Clugston, 2010, p. 112). They were obviously talking about two separate things. This then leads the readers to the symbolism used in the story. The American wants to say anything to convince the girl to go through an abortion, a fact that is not directly mentioned in the story. This is linked to the story’s title Hills Like White Elephants. Hills is usually a symbolism of wanting to escape, while white elephants usually symbolizes something that an individual does not want; in this story, it is the unborn child. Afterwards, the girl takes back her statement and mentions that the hills do not really look like white elephants at all (Clugston, 2010, p. 112). It is a subtle hint that she might want to keep the baby despite the American’s encouragement to abort

Spectrophotometric Assay for Lipase Activity

Spectrophotometric Assay for Lipase Activity Decomposition of human and animals bodies depends on numbers of factors. One of these factors is the presence of bacteria, both endogenous and exogenous of the body. They use the environmental factors to drive the decomposition of the tissues in the body. The various tissues are degraded at different rates by different bacterial cells. As it was seen in the model burial of a pig that is the early stages of decomposition Gram negative bacterial were mostly present in the decaying body. But after 6 7 weeks later the Gram negative bacteria started to decrease as the number of Gram positive bacteria present in the decaying body started to increase. The bacteria produce enzymes which break down any tissue in the body. In the adipose tissue bacteria produces lipases which is secreted in to he tissue and slowly starts to break down the fat. Lipases producing bacterial has been collected from a model burial environment without any environmental factors to see if there is a difference in the activity of the lipase enzyme which are produced by different bacteria species. These bacteria were used in two of the spectrophotometric assay that has been described in the literature. The turbidity assay shows how quickly the lipase enzyme can break down the lipid in the emulsion solution. On the other hand the BALB (dimercaprol Tributyrate) DTNB (5, 5- dithiobis (2-nitrobenzoic acid)) method shows the increase in the product that is produced by the lipase. INTRODUCTION Lipases are found naturally as it is produced by plants, animals and micro-organisms. In the last few decades, the micro-organism production of lipases has been studied for commercial use, which leads to bacterial lipases being studied a great deal. Lipase enzymes breakdown and mobilize lipids which are present within the cell of the organism and the breakdown of lipid is also present in the environment. However there are many questions still unanswered. For example, is the activity of the lipases different when they are produced by different strains or species of bacteria? Hopefully in this research paper, this question will be answered. Bacterial Lipases When bacteria is grown in a surrounding of hydrophobic media, the bacterial cell releases lipase for the breakdown of fats in the environment for a source of energy. Bacteria produce lipases during the late phases of log phases and in the stationary phases. Lipases are hydrolases which hydrolyzes triacylglycerols in aqueous conditions to form fatty acids and glycerol. The reaction releases energy which is used for growth of the bacteria which is why the bacterium produces lipases within these phases. The substrates of the lipases are triacylglycerols which are hydrophobic and the reaction occurs in aqueous condition and this leads to the reaction occurring in lipid-water interface. Some lipases can also catalyze the synthesis of long chain fatty acids. Lipases contains ÃŽ ±/ÃŽ ² fold, which has eight ÃŽ ² sheets in the middle which are parallel except for the second ÃŽ ² sheet and the sheets are surrounded by ÃŽ ± helices. This fold offers a scaffold for the active site in the lipase molecule. The active site or binding site of the lipase molecule is where the interface occurs. This is where the chains of the enzyme are subdivided; at the bottom of the active site is where the ester bond binds to which means this region is hydrophilic. Towards the surface of the enzyme is where the molecule binds to the fatty acids and therefore this region is hydrophobic. Within the ÃŽ ²-sheets there is an area which is highly conserved which is made up of the triad which is a nucleophile and histidine. The nucleophile is made up several amino acids, which are Serine, Cysteine or aspartic acid. The nucleophile is present on ÃŽ ²5 and the histidine is present on ÃŽ ²7. The histidine is the only highly conserved area of the active site/enzyme that d iffers in shape and structure from one type of lipase enzyme to another. Another area of the active site that is important but only present in some type of lipases is the lid. This area is what gives the lipase enzyme the structural explanation of the interface property. When the substrate comes into contract with the lid, it opens the lipid water interface where the substrate binds to for the reaction to occur. Some lipase molecules are only active in the presence of Ca2+ and this is due to the subdivisions of the active site being bound together by the Ca2+ion. The hydrophobic region of the active site leads to less inhibitors that can bind to and inactivate the enzyme. Since lipases are extracellular enzymes, the secretion/production of these enzymes is affected by a number of factors: Nutritional enzymes are produced when the bacteria is in the presence of a lipid environment such as oil, tweens, hydrolyzable esters and triacylglycerols. These are the main sources of lipid but many bacteria can produce lipases in the presence of various sources of substrates. For example Pseudomonas aeruginosa produce lipase in the presence of long chain fatty acids such as oleic and linoleic acid. Temperature the temperature at which maximum production of lipase can occur depends on the optimum temperature for growth of bacteria. The temperature normally ranges from 30 60Â °C, but some can survive at colder or warmer temperatures. Therefore it depends on the type of bacteria in question. pH normally bacterial lipases are active in neutral pH or alkaline pH. However there are a few exceptions like Pseudomonas fluorescens lipase has an optimum pH of 4.8, whereas most bacterial species possess stability over a broad range of pH of 4 10. Effect of ion one type of lipase which is produced by Pseudomonas species is activated by the presence of Ca2+ ion in the environment. Growth of bacteria if the bacterial cell is present in the log phase then the production of lipase is decreased in the bacterial cell. Inhibitors inhibition of lipases does not affect the production or the secretion of the enzyme but affects the activity of the enzyme. There are two types of inhibitors; irreversible or reversible. The reversible inhibitors are split into two types. The first of which are non specific as they bind to the enzyme but not at the active site. When the inhibitor binds to the enzyme, the active site changes and therefore prevents the lipases from binding to the substrate as the structure of the active site has been changed. An example of this type of inhibitor is bile salts. However bile salts can activate some lipases such as the lipase produced by the pancreas. The second type of reversible inhibitors is specific inhibitors as they bind to the active site of the lipase enzyme. They can also be irreversible as the interaction between the inhibitor and the enzyme is so strong that it cannot be broken. An example of this type of inhibitor is boronic acid which can bind to the active site f or a long time but can still be removes leaving the active site unchanged. These types of inhibitors bind to the triad of the active site, which means that when they bind to the triad, the interaction is irreversible. There are three major types of microbial lipases depending on the substrate they bind to. Nonspecific these enzymes act randomly on the lipid substrate molecules which then completely breakdown the molecule. For example with the triglyceride molecule, the enzyme will break the ester in random fashion until the molecule is complete broken down to fatty acids and glycerol. Regiospecific these enzymes only hydrolyze the primary ester bond, these are the C1 and C3 bonds in the triglyceride molecule , which means that when hydrolyzing triglycerides the final products are free fatty acids, 1, 2(2,3)-diacylglyceride and 2-monoacylglyceride. Fatty acid-specific there are some bacteria that only produce this type of lipase and they bind to fatty acids which are then broken down by the lipase. One type of bacteria that can produce lipases that only bind fatty acids is the Achromobacterium lipolyticum. Other bacteria that produce this type of enzyme are Bacillus species which mostly bind to long chained fatty acids. However other bacteria like Pseudomonas species produce lipases that can bind to short or medium length of fatty acids. Staphylococcus aureus can produce a lipase molecule that can bind to unsaturated fatty acids. Lipase in Decomposition The bacteria that are going to be used in the research project are bacteria that were purified from a model burial environment. The bacteria that were present in the model burial environment must have been already been present in the pigs body, which means that all the bacteria that are going to be used are endogenous bacteria that are part of the pigs microflora. The bacteria sample had been taken out of the fluid from the decaying organism in a steel box which was free from all external environmental factors except from oxygen. The sample of bacteria was taken two times a week and then towards the end it was reduced to once a week. It was discovered that at the beginning of the decaying process the bacteria that were present were Gram negative bacteria. However after week 9 the bacteria that were growing in the decaying pig changed from Gram negative to Gram positive. These bacterial cells can release lipases which can break down fats in the body which leads to the formation of adi pocere. Adipocere is made up from a mixture of saturated fatty acids which have been produced during decomposition of the adipose tissue in the body. These adipoceres are formed straight away after death by lipases which are present inside the body. These lipases are mostly produced by the bacteria in the body of the pig which breaks down triglycerides to free fatty acids. If in a suitable environment, bacteria release lipases for hydrogenation of unsaturated fatty acids to its saturated form. Lipase Assays There are two assays that will be performed to find out the activity of the lipase which are present in the solution. The first is based on BALB DTNB method and it uses dimercaprol tributyrate (BALB) and 5, 5 dithiobis (2-nitrobenzoic acid) (DNTB). The lipase enzyme binds to BALB and cleaves it to form an SH group which then binds to DNTB. The product then forms a yellow product which then increases the absorbance which can be measured using a spectrophotometer. The colour intensity is measured at 412 nm; the colour change is proportional to the activity to lipase at to 1:1 ratio. The second assay also uses the spectrophotometer but this time it measures the optical density of the solution instead of measuring the amount of product that is formed. Tributyrin and olive oil is emulsified in the solution which gives a turbid appearance. As the lipase breaks down the lipid in the assay solution, the optical density of the solution decreases which can be measured. The optical density of the solute ion can be measured at 450nm. Both assays measure the activity of the lipase but in two different ways. The first measures the amount of product that is formed while the second measures the breakdown of the substrate. AIMS AND OBJECTIVES Decomposition of human or animal bodies is dependent upon a number of factors. Bacteria which are endogenous (in the body) and exogenous (in the environment) are the key components of decomposition. Different tissues in the body degrade at different rates and are degraded by different bacteria. Previously it has been shown that bacteria in the model burial environment can produce lipases which breakdown the lipids found within the tissues of the body. However it does not tell you if there are different lipases that are secreted by different bacterial cells. Lipase production was demonstrated by using plate assay when lipase breaks down tween 20. Therefore it does not compare the different lipases produced and the activity of different bacterial species. There have been different spectrophotometric assays that have been described in the literature to calculate the activity of lipase enzymes, but only two of these will be used. The bacteria that is going to be used in the assay has been purified from fluid from a decaying pig in a steel box which is free from all external environmental factors expect oxygen. Two assays are going to be preformed to find the activity of lipase, the first one similar to the BALB DTNB method. Lipase forms a SH group on BALB which then binds to DTNM to give a yellow product. The amount of product that is formed in a solution is related to the activity of lipase in a 1:1 reacting ratio which is a direct measurement of the activity. The colour change is measured at 420 nm. The second assay is also measure the change in the solution but this time it measures the decrease of the substrate that is left in the solution. It measures the density of the solution, as the substrate (olive oil) is denser than the product. The density is measured 450 nm. The decreased of the substrate is related to the activity of lipase. At first before anything can be done we need to see if the bacteria cells produced lipase is by growing them in a plate which contains Tween 80. If the Tween is broken down then the bacterial cell produces lipase. MATERIALS AND METHODS The bacterial strains that were given to me were extracted from fluid from a pig that was decaying in a steel box which had a controlled environment that was free from all external environment factors expect fresh air. Bacterial Media The bacterial strains were grown in half nutrient agar which was made from 2.6g of nutrient broth (OXOID, Basingstoke, England) and 4.8g of Agar bacteriological (OXIOD) in 400ml of water which was autoclaved and then poured in to 20ml Petri dish. The bacterial strains were plated and left in a 30Â °C incubator overnight. After the bacteria were grown on just half nutrient agar, they were then grown on half nutrient agar with 4ml of sterile Tween 80 (SIGMA ALDRICH, UK) and 400Â µl of 10% of CaCl2 (scientific equipment, Loughborough, England). Again the plates were placed in a 30Â °C incubator. The bacterial strains were also grown in minimal medium agar which contained 2.8g of Potassium Hydrogen Orthophosphate (BDH Laboratory Supplies, Poole, England), 1.2g Sodium Dihydrogen Orthophosphate (BDH LS) and 0.04g of Magnesium Sulphate (BDH LS) in 200ml of sterile water and 2.4g of Agar bacteriological. After the solution came out of the autoclave, 2ml of Tween 80 was added and 200Â µl of 10 % CaCl2. For the bacterial strains to be used in spectrophotometric assay, the strains had to be grown in liquid media. The bacterial strains were grown in two different types of media, Tryptic Soy Broth and Minimal Medium. The Tryptic Soy Broth (TBS) was made from 30g/L Tryptone Soya Broth (OXIOD) which was autoclaved. After the bacteria were added to the media, the bottle was placed in a shaking incubator at 37Â °C over night. The Minimal Medium contained 14g/L of potassium hydrogen orthophosphate, 6g/L sodium dihydrogen orthophosphate and 0.2g/L of magnesium sulphate. 100Â µl of Tributyrate (SIGMA ALDRICH) was added to 10ml of the Minimal Media. The bacteria were added to the media and then placed in a shaking incubator at 37Â °C over night. Sample Solutions After the bacteria are left to grow, the media is used to make up three different samples of bacteria to use in both of the assays. The first sample is purified bacterial strain from the media and this was obtained when 1ml of the media was placed in a sterile eppendorf tube which was then centrifuged at full speed for 2 minutes. The supernatant was replaced with 500Â µl of 150mM of CaCl2 and 500Â µl of 200mM of Tris buffer (12.11g of Trizma base in 150ml of water and then 0.1M of HCl was added to make the pH of the solution 8, this to make 0.5M Tris Buffer which was then diluted to make 200mM solution) (SIGAM ALDICH). The second sample was done in the same manner but instead of adding Tris buffer and CaCl2 to the pellet, PBS (Phosphate buffered saline) solution is utilized to re-suspend the pellet and 2ml of the media solution is used. Each suspension was transferred in to a different Bijou Bottle which is kept on ice. The suspension in the Bijou Bottle is sonicated twice for 30 seconds at 30W. The last sample was made when the media solution is filtered with the use of a sterile syringe and sterile 0.2Â µm pore syringe filter and placing the filtered solution into a sterile universal bottle. 3ml of the media was only filtered. The samples were ready for the assay and two different that were used. They both measured the absorbance of the solution at different wavelengths. One measured the turbidity of the solution while the other looked at the change in the absorbance of the solution. Turbidity Assay For the turbidity assay an emulsion solution is made and it is made from 100mM of Tris buffer (4.975ml), 50mM of CaCl2 (4.975ml) and 50ml of lipid source (either olive oil or Tributyrate or both). The solution was sonicated for 3 minutes at 40W. The solution is left in a water bath until it is used for the assay. The emulsion solution is used in three different ways as the assay was performed in a cuvette, Petri dish or 96 well plate. When done in a cuvette, 40mg of low melting point agarose (SIGMA ALDRICH) is added and the boiled before sonication. The agarose stabilises the emulsion. If the assay was done in a 96 well plate, then no agarose is necessary. The last test that is performed is in 20ml plates; 20ml of the emulsion solution is made up with 80mg of agarose to made a solid media (INVITROGEN, Paisley, UK) which is then boiled before and after sonication. For the 96 wells plate, 200Â µl of the emulsion solution was placed in each well and then 20Â µl of the sample solution was added. As soon as the sample was added the absorbance is measured at 450nm to measure the optical density of the solution. The absorbance was then measured every 15 minutes up to 60 minutes. Here the samples that were used were grown in the Minimal Medium. The lipid source in this part of the assay was 25Â µl of olive oil and 25Â µl of Tributyrate in 10ml of the emulsion solution. For the assay that was done in the cuvette 1L of the emulsion solution was added to a micro cuvette and 100Â µl of the sample solution. The absorbance was also measured at 450nm as soon as the sample is been added and then every 5 minutes up to 45 minutes. The lipid source is 50Â µl of olive oil in 10ml of emulsion solution. For the plate assay after the solution was boiled for the second time, the solution was poured in to a plate for the agarose to set. After the agarose was set, wells were made in the agarose using a hollow punch about 8mm in diameter which was filled with 10Â µl of the sample solution and the plate was left at room temperature over night. In 20ml of the emulsion solution the lipid source was 50Â µl of each olive oil and Tributyrate. Colour Assay (BALB DNTB Method) The second assay measures the absorbance change in the working solution. The working solution is made from BALB (SIGMA ALDRICH) and DNTB (SIGMA ALDRICH) and Tris buffer solution. The working solution was made from 1 ml of BALB is added to 17.5ml of 0.5M of Tris Buffer at pH 8.5 and 625mg of DNTB. 150Â µl of the working solution is added to the well after adding 150Â µl of water. To this 10Â µl of the sample was added. When the assay was done in 96 well plate the absorbance was measured after the sample was added at 405nm and then every 10 minutes for 30 minutes. When the assay was done in a cuvette, at first 400Â µl of water was placed in the cuvette then 380Â µl of the working solution was added to the water. Then the 20Â µl of the working sample was added into the cuvette. The absorbance was the measured at 412 nm for the 20 minutes. The reason why there is a difference in the wavelength in which the absorbance is measured is due to the plate reader not being able to read the absorbance at 412nm. For this assay the samples that were used were prepared from the bacteria that were grown in TSB. RESULTS When the bacteria colonies were grown on the agar plate which had Tween 80 and CaCl2, around the colonies there was the presence of halos or the colonies has a halo this can be seen in figure 1a. The arrow shows the halo colonies of the bacteria species. The bacteria colonies that were placed on other plates was not as clear as 16C but the halo can only be seen when the plates are held up by the light (result not shown). Turbidity Assay The first assay that was done was the turbidity assay in a cuvette, the optical density of the solution did not increase or decrease, and it just stayed the same. But when the assay was done in the 96 well plate the optical density increased when the bacteria were added to the well, and then decrease and keep decreasing even after 60 minutes (figure 2a). Then the filtered media was added to the emulsion solution in the 96 well plate, the optical density again decreased. However not all the bacteria were filtered to see if there was a decrease in the optical density (figure 3). Only some of the bacteria were used to see if it was an enzyme that was decreasing the optical density and not the bacterial cells. However the general result showed a decrease in the optical density except for 2 bacterial strains (1A and 4A) which showed an increase in the optical density after 30 minutes and then it optical density again. Then the bacteria cell free lysates were added to the welled plate and the same result appeared as the optical density levels decreased once again. The bacteria that were used were the same bacteria that were used in the filtered part of the assay (figure 4). After 45 minutes the optical density is starting to level off. The gradient of the line for all the bacteria strains are the same as they all decrease at the same rate expect for bacteria strain 5 which has flatter gradient than the rest. For the plate test in the turbidity assay, the bacterial solution in the well was not present and no zone of clearance was noticeable in any of the plates (figure 1b). Only one of the plates is shown in the figure and the rest of the plates looked the same as no zone could be seen. Colour Assay (BALB DNTB Method) In the BALB-DNTB method, the absorbance increases when bacteria strain 6 was added to the working solution in a cuvette and measured for 20 minutes. The increase was slow for the first 10 minutes and then increased at a faster rate for the next 10 minutes, figure 5. When the assay was done in the welled plate, the absorbance increases for all the strains but some increase more than others. For example strain 5 increased from 4.204 to 4.412 while strain 1 only increased from 4.241 to 4.265. This is shown in a table in figure 2b. When only the media in which the bacteria grew in was added as the sample, the absorbance also increased for most of the bacterial strains but not as much as when the bacterial cells were added. For some of the strains the absorbance decreased. For example in strain 1 there was a decrease from 4.241 to 4.235, figure 2c. The same happened when the content of the bacterial cell was added to the working solution. But when the absorbance increased, the increase was bigger than the increase when media was added (figure 2d). However there were still some strains in which the absorbance still decreased in 20 minutes but the absorbance increased from 0 to 10 minutes and then decreased from 10 to 20 minutes. Figure 1, (a) the plate has been plated with strain 16C (left) and 16B (right); the halo can be seen clear by the arrow which is the colonies of bacteria 16C. However the halo can not be seen clearly in the colonies of bacteria. (b), the plate contain solid emulsion solution with well which contain lipases from different bacteria, and there is no presence of zone of clearance from any of the well. There were 3 plates in total and all look the same (only one is shown) but the well had different lipases from different bacteria. Figure 2, A is a table that shows the optical density change when bacterial was added to emulsion solution for the turbidity assay. The optical density decreases when the bacterial cells were added to the emulsion solution. The next 3 tables are showing the absorbance change when the strains were added to the working solution for the colour BALB-DNTB method, (B) has bacterial cells added to the working solution; (C) has only filtered media, which had bacteria growing in, was added and lastly (D) had bacterial cells free lysates added. In the colour assay the absorbance increased in all three cases. DISCUSSION Bacteria produce lipases that can break down or hydrolyse lipid molecules such as fats and oils. They produce lipases in the log phase of growth when there is a high level of lipid source for energy. There are different lipases which can break down different lipid molecules. The bacterium produces lipases to break down lipid for energy as adequate amount energy is present in lipids. As most of the lipids cannot cross the cell membrane, the lipid has to be catabolised into smaller lipid molecules which can then enter the cell where it is broken down further. Lipases from bacteria are studied for industrial uses. Here it was studied to see if the lipases that were produced from different bacteria are different and if there was any variation in the activity of the lipases. When the bacterial cells were grown on agar plate without any Tween 80 the bacterial colonies do not have any halos or precipitate around the colonies. But when some of the bacteria were grown in agar that contained Tween 80 and CaCl2 the colonies had halo colonies 3 to 8 days after they were inoculated. In the past Tween has been used for lipase activity to see if the bacteria produce lipase. If lipases are produced then it binds to the Tween and breaks the Tween down to fatty acids. The fatty acids then bind to the Ca in the media which forms crystals. These crystals then become soluble in the media which can then be seen by eye as halos. Some of the colonies had halos which meant that the cell produced lipases. Figure 6, the turbidity plate assay should have looked like this but what the figure 1b shows. There the one of clearance can be seen very clearly where as in the plate in figure 1b there are no clearing at all what meant the assay did not work at all. The turbidity assay that was done is the plate which showed no zone of clearance, it should have had zone of clearance around the well which contained the sample of bacteria. The bacteria in the wells should have diffused out of the well and in to the agarose media in which the bacteria should have released lipases to break down the olive oil and Tributyrate. When the lipids were broken down the media would have become clear. The plate should have look like figure 6 from, the zone of clearance is shown very clearly. The other assay that did not work was the same assay that was done with the cuvette. This is when the absorbance levels did not decrease but just stayed the same. The absorbance levels should have decreased and the reason in why this did not occur is not known. It might have been due to the stability of the solution as the agarose must have been concentrated which meant that the bacteria solution was not able to diffuse through the media. The concentration of agarose might be the problem because when agarose was not added like in the 96 well plate part of the assay, the absorbance of the emulsion solution decreased. This was due to the emulsion solution being turbid by lipid in the solution when sonicated, when the bacteria sample was added the optical density increased slightly as the bacteria cell scatter the light which leads to the increase in the optical density absorbance levels. The bacteria cell then releases lipase in the solution or lipase that are inside the cell break down the lipid in the emulsion solution which then leads to the decrease in the level of lipid in the emulsion solution which then means that less light is scattered. The well plate assay was done to 3 different type of sample solution, one of which contained bacteria cell, one of which contained the filtered media solution and the last contained the bacteria cell free lysates. The bacterial cells were used to see if the bacterial cell produced lipases. The filtered media was used to see if the bacterial cell released lipase in to the media and if it was in fact the lipase that was decreasing the absorbance and not anything else. The bacteria content was used after the bacteria cell were sonicated for one minute, to use all the lipases that had been produced by the bacterial cell but not secreted. As not all the bacteria cells release the lipase in to the media and sometime the lipid molecule is too big to cross the cell membrane and wall of the bacteria. To see if there are any differences in the activity of the different lipases which are produced by different bacterial cells, cannot be done by adding the sample to the emulsion solution as different concentration of lipase must have been in the sample for each of the strains. In order to make the test fair, the amount of bacterial cell and the lipase concentration must be the same for each of the bacterial strain. But still it might be a fair test as some of the bacterial cells can still divide inside the emulsion solution and then increase the concentration of lipases. The lipases produced by the bacteria are produced in the log phase. The same can be said for the BALB-DNTB method. This assay is not like the other assay because the absorbance does not decrease but increase. This is due to the lipase bind to the BALB in which is cleaved to form a SH group. The SH group then binds to DNTB which is in excess in the working solution, to form a yellow substance. The complex then absorbs light hence increasing the level of absorbance. The bind of the BALB with the new SH group binds to the DNTB in a one to one reacting ratio, this means that increases is absorbance is proportional to the reacting activity of the lipase. When bacterial cells were mixed to the working solution the absorbance for most of them increase. This meant that lipases that were present in the well were cleaved BALB. The same thing also occurred when filtered media was added to the working solution but the increase were small and this must be due to the fact that not a lot of lipases were released by the bacterial cells in to the media solution. However, when the bacterial cell free lysates is added not all of the absorbance levels increase but in fact some of them decrease and then increase. It may mean that the lipases need time to start working since they had been on ice before the experiment. To see if this was true, the test needs to be done again but for a longer period of time. In the cuvette test, only one strain, it was used when the first assay was done it had the largest change in absorbance. It was used to see a general increase of the solution over 20 minutes and the absorbance was measured every minute to see the turning point when the rate of enzymatic activity change from being slow to a steady normal rate. The graph in figure 5 shows that the rate was slow during the first 10 minute this meant the bacteria cell needed to adapt to the new environment before the activity of the enzyme can to back to normal. If the test was done longer then the graph would start to level due to the substrate concentration starting to decrease. From the results, there is not enough evidence to conclude that there any differences in the activity of the different strains of lipase. To see if it is true then the both of the a

Moral Actions Essay -- Philosophy Essays

Moral Actions Honesty and deceit. Compassion and Neglect. Benevolence and malevolence. All these represent the extremes in the spectrum of morality. From the general societal viewpoint, the former represents the attitudes which should be admired, rewarded and emulated, while the latter represents the attitudes which should be abhorred, punished and discouraged. Now philosophers, not being satisfied with leaving things well enough alone, endeavour to discover why this is so. Why do we admire acts of kindness? Why do we loathe acts of malice? It is generally thought that the crux of this question of morality has to do with the magnitude of selfishness accounted for in the acts and thoughts of individuals. If we can think of selfishness as an empirical property, honesty, compassion, and benevolence are acts and attitudes that involve much less selfishness than their moral opposites. This realization, of course, does not answer the question we are considering, it merely pushes it back one metaphysical level. So the revised question should be this: When is selfishness morally acceptable, and when is it not? Nietzsche, in proposing that selfishness is, in a sense, completely free of moral blame at all, comes to a conclusion that is completely opposite to the rest of the philosophers that we have studied. We shall see that Nietzsche is probably on the right track, and that selfishness is a faulty gauge of the morality of an action, and that morality is simply an illusory concept created by the individuals of society to prevent harm to themselves. We have all seen it before. The African savanna. A cheetah. A pack of grazing gazelles. The cheetah stealthily approaches toward the pack of grazing gazelles. N... ... of when selfish acts are morally permissible, we have first established that all sane actions are selfish in origin, and therefore, selfishness cannot be used as a measurement of morality. Secondly, the standard of morals which we use to gauge themorality of an action is based on our own selfish desire for personal power. As established by Nietzsche, actions done in the pursuit of personal power are natural, and therefore, from our own viewpoint, these actions are never objectionable. It is only when seen from another's perspective that these actions can be seen to be despicable because it threatens their personal pursuit for power. Therefore, the actions that others find objectionable are the actions performed by us that do not involve stealing personal power away from another. In this case, there is no definite set of morals that one can measure their actions to.