Peroxisomes research Article Example | Topics and Well Written Essays - 1250 words

Peroxisomes research - Article Example Luciferase measurement The genomic DNA was amplified using PCR 500 bp upstream region of Act1 Cta1, iPex3A and iPex3B open reading frames. The PCR fragments were inserted upstream of Luciferase reporter gene in pLUC plasmid. The promoter-pLUC plasmids were transformed into wild-type yeast via the PEG - LiAC transformation method. The Transformants were plated on YPD plates with kanamycin. Each colony of the transformant was grown in 5ml YPD for 18 hours at 30C. The cells were spin down in a centrifuge. The cells were resuspended in 1mL low fluorescence media (YNB). This sample was divided into two parts. To one sample 100uls of 0.003% hydrogen peroxide was added. The tubes were incubated at 28C for 30 minutes. The cells were spin down. The cells were washed 3 times with 1ml 1XPBS. The final pellet was resuspended in 500 ls of 1X PBS. The cells were separated into 10,000 cell aliquots. One ml lysis reagent to the cells and incubated for 5 mins. The luciferase assay reagent was added to lysed cells. The int ensity of light emitted from sample was measured with a luminometer. Statistical analysis: Each sample were measured six times and standard deviation was determined. Further, t-test were carried out to signify the difference in the values. Results: To determine the role of iPEX3A and iPAX3B in peroxisomal function set of experiments were carried out. In first sets of experiments accumulation of H2O2 were determined in different mutant having single mutation in cat1, ipex3A and ipex3B. Table 1 displays the results of H2O2 treatment on these three mutants along with wild type (positive control) cells. There was significant increase (p

Internet Marketing Project Essay Example | Topics and Well Written Essays - 5250 words

Internet Marketing Project - Essay Example In the onset of advanced information technology, internet marketing has become an integral part of its existence. Marketers use this as an opportunity to market their products and service offerings. The purpose of this report aims to create a comprehensive web traffic and marketing plan to promote and develop the Facebook group website--MarketNet. The information and ideas used in this report are coming from reliable journal articles and relevant textbooks. All ideas especially in the section of web market plan include important marketing concepts that are practically obtained from various marketing studies. Central Queensland students are increasing in number and they are coming from local and international. The increase of students ensures increase of website traffic at Alexa, the Central Queensland University website. It is found that from demographics, psychographic, geographic and behavioral analysis, students tend to appreciate the value of website for them to use relevant information based on their needs. The Web Traffic Plan is a comprehensive strategic move to ensure successful implementation of marketing objectives prior to increase web traffic for MarketNet. The plan started with different marketing objectives then followed by strategic moves based on the principles of extended marketing mix. Finally, the proponent tries to indicate important activity measures that will help ensure that the marketing objectives are remarkably achieved. ... in traditional approach of sharing information about their product or service offerings, but specifically on employing the best strategies by using the full potential of internet. Today, marketing finds its way through social network sites. It tries to implement this by ensuring remarkable traffic. However, social network sites need also to promote themselves. In the case of Facebook, competition is very tough. There are various social network sites that try to compete in order to become a cut above the other. Thus, the goal for each of them especially for Facebook is to market this site. One way of doing this is to ensure high traffic and substantial marketing plan. 1.2 Aims This report aims to create a comprehensive web traffic and marketing plan to promote and develop the Facebook group website--MarketNet. 1.3 Scope This report includes the following: target audience analysis of the marketing students at Central Queensland University, and web traffic plan for the promotion and dev elopment of Facebook group website MarketNet. The information and ideas used in this report are coming from reliable journal articles and relevant textbooks. All ideas especially in the section of web market plan includes important marketing concepts that are practically obtained from various marketing studies. 2.0 Target audience analysis (Central Queensland University marketing students ) In order to help analyze this part, the proponent uses database graph from Alexa which can be found from the following website: http://www.alexa.com/siteinfo/cqu.edu.au. In the following subsections, parts of the target audience analysis include discussions in line with demographics, psychographics, geographic and behavioral. 2.1 Demographics Table 1. Search Traffic of Central Queensland University’s